| Baza: |
Plazmidów |
| Nr w bazie: |
1654 |
| Nazwa plazmidu: |
pPDB4.32 |
| Gatunek: |
Escherichia coli |
| Szczep: |
DH5α |
| Genotyp: |
recA1, endA1, gyrA96, thi-1, hsd R17(rK-mK+), supE44, relA1, deoR, Δlac(ZYA-argF), U169,[φ80Δ(lacM15)]
|
| Sekwencja plazmidu: |
non available
|
| Konstrukcja: |
pGAD424 (shuttle vector E. coli/ S. cerevisiae,oriMB1/2μ, leu2, GAL4AD, ApR; Fields and Song, 1989)derrivative with EcoRI/SalI fragment encoding KfrB from R751 with deletion of N-terminal part
|
| Plasmid properties/Comments: |
|
| Mapa plazmidu: |
 |
| Markery selekcyjne: |
AmpR |
| Stężenie antybiotyku: |
|
| Wielkość plazmidu: |
~6,8kb |
| Origin of replication/Incompatibility group: |
|
| Host range: |
|
| Inne elementy genetyczne: |
|
| Właściwości szczepu/komentarz: |
Fragment carrying kfrB gene was previously amlified and cloned into pGAD424 vector (shuttle vector, see references: Adamczyk, M., 2002, Ph.D.Thesis - pMAB424.4). pMAB424.4 was cleaved with XmaIII and blut-ended with Klenow fragment of polymerase then liga |
| Autor: |
Patrycja Dolowy |
| Dostępność: |
non restricted |
| Patent: |
|
| Osoba deponująca: |
Patrycja Dolowy |
| Instytucja/wydział: |
|
| Data zdeponowania: |
2004-11-18 10:58+01:00 |
| Referencje: |
1) Fields, S and Song, O. (1989) A novel genetic system to detect protein-protein interaction. Nature 340: 245-246; 2) Adamczyk, M.(2002) The role of KfrA protein in the stable inheritance of IncP-1 plasmids. Ph.D. Thesis IBB PAN, Warsaw, Poland |