α β γ δ ε ζ η θ ι κ λ μ ν ξ ο π ρ Σ σ τ υ φ χ ψ ω Δ Γ Ω Σ Λ Ψ Φ Ξ Θ Π °C Greek letters

Central Collection of Strains (IBB)

Base: Yeast strains
Institution Code: IBBPAS
Collection Code: ColIBB
Number in base: 4002
Species: Saccharomyces cerevisiae
Strain: SC30
Genotype: MAT α his3Δ1 leu2Δ0 lys2Δ0 MET15 ura3Δ0 dpb2::kanMX4 [pGJ2(pRS313 HIS3 DPB2)]
Plasmid: pGJ4= pRS313 HIS3 dpb2-GJ4
Plasmid map:
Selectable Markers: Leu Lys Ura kanMX4
Antibiotic concentration:
Construction: Derivative of no.2893D (SC11); SC11 strain was transformed with pGJ4, transformants were picked twice throught 5-FOA containing plates to lose pMJDPB2 (URA3) plasmid
Strain properties/Comments: strain grows well at 23-37oC, pGJ4 plasmid is isogenic with pGJ2(no.2530P) but instead DPB2 allele contains dpb2-GJ4 allele; dpb2-GJ4 is coding mutated Dpb2p variant containing single subtitiution Met572Lys
Author: Malgorzata Jaszczur
Availability: restricted
Patent:
Depositor: Malgorzata Jaszczur
Deposition date: 2009-03-04 18:03+01:00
References: