| Base: |
Yeast strains |
| Institution Code: |
IBBPAS |
| Collection Code: |
ColIBB |
| Number in base: |
4003 |
| Species: |
Saccharomyces cerevisiae |
| Strain: |
SC32 |
| Genotype: |
MAT α his3Δ1 leu2Δ0 lys2Δ0 MET15 ura3Δ0 dpb2::kanMX4 [pMJ101(pRS313 HIS3 dpb2-101)] |
| Plasmid: |
pMJ101= pRS313 HIS3 dpb2-101; old plasmid name pGJ5 |
| Plasmid map: |
 |
| Selectable Markers: |
Leu Lys Ura kanMX4 |
| Antibiotic concentration: |
|
| Construction: |
Derivative of no.2893D (SC11); SC11 strain was transformed with pMJ101, transformants were picked twice throught 5-FOA containing plates to lose pMJDPB2 (URA3) plasmid |
| Strain properties/Comments: |
strain grows well at 23 and 30 oC, slower growth at 37oC, pMJ101 plasmid is isogenic with pGJ2(no.2530P) but instead DPB2 allele contains dpb2-101 allele; dpb2-101 is coding mutated Dpb2p variant containing single subtitiution Lue284Pro; dpb2-101 mutants |
| Author: |
Malgorzata Jaszczur |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Malgorzata Jaszczur |
| Deposition date: |
2009-03-04 18:03+01:00 |
| References: |
Jaszczur et al. 2008 Genetics 178: 633-647. |