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Central Collection of Strains (IBB)

Base: Yeast strains
Institution Code: IBBPAS
Collection Code: ColIBB
Number in base: 4055
Species: Saccharomyces cerevisiae
Strain: SC282
Genotype: MAT α his3Δ1 leu2Δ0 lys2Δ0 MET15 ura3Δ0 dpb2::kanMX4 rev3::CaURA3[pMJ101(pRS313 dpb2-101 HIS3 )]
Plasmid: pMJ101 = pRS313 dpb2-101 HIS3
Plasmid map:
Selectable Markers: Leu Lys kanMX4
Antibiotic concentration:
Construction: Derivative of 4003D (SC32); transformed with rev3::CaURA3 disruption cassette, integration of rev3::CaURA3 cassette to the chromosome leads to the deletion of entire REV3 ORF; disruption confirmed with PCR
Strain properties/Comments: strain express mutator phenotype when comparing with DPB2rev3 strain; rev3::CaURA3 mutations in dpb2-101 strain decreases rate of mutagenesis
Author: Malgorzata Jaszczur
Availability: restricted
Patent:
Depositor: Malgorzata Jaszczur
Deposition date: 2009-03-04 18:03+01:00
References: