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Central Collection of Strains (IBB)

Base: Yeast strains
Institution Code: IBBPAS
Collection Code: ColIBB
Number in base: 4061
Species: Saccharomyces cerevisiae
Strain: SC382
Genotype: MATa trp1-289 his7-2 leu2-Δ::kanMX4 ura3-Δ ade2-1 lys2-ΔGG2899-2900 CAN1 pol2-4 dpb2::pKF117dpb2-100(CaURA3, HIS3)rev3::LEU2
Plasmid:
Plasmid map:
Selectable Markers: trp, lys, his, ade, kanMX4(G418), CAN1
Antibiotic concentration:
Construction: derivative of no. 4038 (SC148); SC148 was transformed with rev3::LEU2 disruption cassette; inetgration of rev3::LEU2 cassette leads to the deletion of the entire REV3 ORF; disruption confirmed by PCR
Strain properties/Comments: strain grows well at 23oC, slowly at 30oC and does not grow at 37oC; strain express mutator phenotype; mutation frequencies can be measured at different genetic loci; trp1-289,ade2-1 allow to measure base substitution; his7-2 lys2-ΔGG2899-2900 allow
Author: Malgorzata Jaszczur
Availability: restricted
Patent:
Depositor: Malgorzata Jaszczur
Deposition date: 2009-03-04 18:03+01:00
References: