| Base: |
Yeast strains |
| Institution Code: |
IBBPAS |
| Collection Code: |
ColIBB |
| Number in base: |
81 |
| Species: |
Saccharomyces cerevisiae |
| Strain: |
WCG4a ump1 rev3 |
| Genotype: |
MAT a ura3 leu2-3,112 his3-11,15 rad5-535 CanS GAL2 ump1::kanMX4 rev3::HisG |
| Plasmid: |
|
| Plasmid map: |
 |
| Selectable Markers: |
ura leu his kanR |
| Antibiotic concentration: |
|
| Construction: |
Into UMP1 place cassette with G418 resistance gene was inserted. Instead of REV3, hisG::URA3 was inserted, followed by loosing URA3 on 5-FOA.
Correctness of the construction was confirmed by PCR |
| Strain properties/Comments: |
isogenic to the strain WCG4a |
| Author: |
Adrianna Skoneczna |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
|
| Deposition date: |
20.12.2001 |
| References: |
|