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Central Collection of Strains (IBB)

Base: Plasmid collection
Number in base: 106
Plasmid name: pMH123
Species: E.coli
Strain: DH5α
Genotype: supE44 Δ lacU169(φ80 lacZ Δ M15)hsdR17 recA1 endA1 gyrA96 thi-1 relA1
Plasmid Sequence:
Construction: vector pBR322, fragment 3,45kb PvuII containing gen cbl from E.coli with cam (0,8kb) from pCM7(Pharmacia-LKB) inserted in HindIII site of OFR cbl, cloned in SspI/PvuII-PvuII/EcoRV
Plasmid properties/Comments:
Plasmid map:
Selectable Markers: Amp cam
Antibiotic concetration:
Plasmid size: 7,4kb
Origin of replication/Incompatibility group:
Host range:
Other genetic elements:
Strain properties/Comments:
Author: R.Iwanicka-Nowicka
Availability: restricted
Patent:
Depositor: M. Witkowska
Institution/Department:
Deposition date: 11.03.2002
References: