Baza: |
Plazmidów |
Nr w bazie: |
2556 |
Nazwa plazmidu: |
pGJ101 |
Gatunek: |
Escherichia coli |
Szczep: |
DH5α |
Genotyp: |
φ80dlacZ Δ M15,recA1,endA1,gyrA96,thi-1,hsdR17(rk-,mk+),supE44,relA1,deoR, Δ(lacZYA-argF)U169 pGJ101
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Sekwencja plazmidu: |
TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCA
CAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTG
TTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGC
ACCATAAACGACATTACTATATATATAATATAGGAAGCATTTAATAGACAGCATCGTAAT
ATATGTGTACTTTGCAGTTATGACGCCAGATGGCAGTAGTGGAAGATATTCTTTATTGAA
AAATAGCTTGTCACCTTACGTACAATCTTGATCCGGAGCTTTTCTTTTTTTGCCGATTAA
GAATTAATTCGGTCGAAAAAAGAAAAGGAGAGGGCCAAGAGGGAGGGCATTGGTGACTAT
TGAGCACGTGAGTATACGTGATTAAGCACACAAAGGCAGCTTGGAGTATGTCTGTTATTA
ATTTCACAGGTAGTTCTGGTCCATTGGTGAAAGTTTGCGGCTTGCAGAGCACAGAGGCCG
CAGAATGTGCTCTAGATTCCGATGCTGACTTGCTGGGTATTATATGTGTGCCCAATAGAA
AGAGAACAATTGACCCGGTTATTGCAAGGAAAATTTCAAGTCTTGTAAAAGCATATAAAA
ATAGTTCAGGCACTCCGAAATACTTGGTTGGCGTGTTTCGTAATCAACCTAAGGAGGATG
TTTTGGCTCTGGTCAATGATTACGGCATTGATATCGTCCAACTGCATGGAGATGAGTCGT
GGCAAGAATACCAAGAGTTCCTCGGTTTGCCAGTTATTAAAAGACTCGTATTTCCAAAAG
ACTGCAACATACTACTCAGTGCAGCTTCACAGAAACCTCATTCGTTTATTCCCTTGTTTG
ATTCAGAAGCAGGTGGGACAGGTGAACTTTTGGATTGGAACTCGATTTCTGACTGGGTTG
GAAGGCAAGAGAGCCCCGAAAGCTTACATTTTATGTTAGCTGGTGGACTGACGCCAGAAA
ATGTTGGTGATGCGCTTAGATTAAATGGCGTTATTGGTGTTGATGTAAGCGGAGGTGTGG
AGACAAATGGTGTAAAAGACTCTAACAAAATAGCAAATTTCGTCAAAAATGCTAAGAAAT
AGGTTATTACTGAGTAGTATTTATTTAAGTATTGTTTGTGCACTTGCCTGCGGTGTGAAA
TACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGAAATTGTAAACGTTAATATTTT
GTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAAT
CGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGT
TTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGT
CTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAG
GTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGG
AAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGC
GCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCC
GCTACAGGGCGCGTCGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATC
GGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAGGGGGGATGTGCTGCAAGGCGATT
AAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATT
GTAATACGACTCACTATAGGGCGAATTGGAGCTCGACATAACACCGCAGGAAGAAAAGAA
GAAAGTTGAACTAAGAAATCGAAAAGCAGAGAATATGGATCTTCACCCGAGCAGGAAGAT
GCATATTAAGGAACTTCTACACTCTTTGAACTTAGATATGTGTAATGATGAGGAGGTTTA
TCAGAAAATATCACTTTACTTACAGAAAAATGAGGAAAGCAGGACTTCAGTGGGTGCCTC
GCAACAAAACCACGTAGATATCGATATAAACTCCCTAAAACGCTATCTACAAAATATTGA
AAAGAAGGCACGTCAAAAAAGTGCCATCGATAAACAAAAAAAGAATCAAGCTCGTATATA
TCAATGGAATACTCAATCCTTCTCCGAAATAGTACCCTTATCAGCTGGAAATATCTTATT
CAAAAGGGAACCCAATCGCTTATGGAAACGTTTACAAAATGGAATAAGTGTATTTTTAGG
TTCAAACGGAGGAGGAAAAAAGAGTAAAACTACTAAAAAAGTACTTCAAGGGAATAACAT
ATTGTTACACTCATTAGAAAACAATAAGGATATGACCCTGTCAAACAACTTTGATCACAG
TGTCTTTAACATCAATTTTACGGACTTATTTGGCGTTATTAATGCATCTGGGTCCCCACC
AGATAGAGTTTTGAACGAAATTAATGAGATCGAATTAAAAGGTTGGAAATGTGTGGGGAA
CTTATACGATAATAATAAAATTGTAGTTTTTCAAAGTAGCAATCCACTCTTAGAGGATAC
GAAGATTCCTCAAAAGTCTTTCACTAATTCAAAGAGATTCCTTATTTCACTCTCAGCATT
GCTGGCCTCCTTTTTTGCATATTATAGATACCGTCTATCTCAACGTCAAGAATCAAAGAA
ATGATTTTTTTCTTTCTTGTATATAACAACGTTATACATAGAAACAAATAACTACTCATC
ATTTTGCGAGACATATCTGTGTCTAGATTACCCGGGAAAAACTTGATTAGGGTGATGGTT
CACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGT
TCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATT
CTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTT
AACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCCTGATGCGGTA
TTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATCGACCCTCGAGGAGAACTT
CTAGTATATCCACATACCTAATATTATTGCCTTATTAAAAATGGAATCGGAACAATTACA
TCAAAATCCACATTCTCTTCAAAATCAATTGTCCTGTACTTCCTTGTTCATGTGTGTTCA
AAAACGTTATATTTATAGGATAATTATACTCTATTTCTCAACAAGTAATTGGTTGTTTGG
CCGAGCGGTCTAAGGCGCCTGATTCAAGAAATATCTTGACCGCAGTTAACTGTGGGAATA
CTCAGGTATCGTAAGATGCAAGAGTTCGAATCTCTTAGCAACCATTATTTTTTTCCTCAA
CATAACGAGAACACACAGGGGCGCTATCGCACAGAATCAAATTCGATGACTGGAAATTTT
TTGTTAATTTCAGAGGTCGCCTGACGCATATACCTTTTTCAACTGAAAAATTGGGAGAAA
AAGGAAAGGTGAGAGGCCGGAACCGGCTTTTCATATAGAATAGAGAAGCGTTCATGACTA
AATGCTTGCATCACAATACTTGAAGTTGACAATATTATTTAAGGACCTATTGTTTTTTCC
AATAGGTGGTTAGCAATCGTCTTACTTTCTAACTTTTCTTACCTTTTACATTTCAGCAAT
ATATATATATATTTCAAGGATATACCATTCTAATGTCTGCCCCTATGTCTGCCCCTAAGA
AGATCGTCGTTTTGCCAGGTGACCACGTTGGTCAAGAAATCACAGCCGAAGCCATTAAGG
TTCTTAAAGCTATTTCTGATGTTCGTTCCAATGTCAAGTTCGATTTCGAAAATCATTTAA
TTGGTGGTGCTGCTATCGATGCTACAGGTGTCCCACTTCCAGATGAGGCGCTGGAAGCCT
CCAAGAAGGTTGATGCCGTTTTGTTAGGTGCTGTGGGTGGTCCTAAATGGGGTACCGGTA
GTGTTAGACCTGAACAAGGTTTACTAAAAATCCGTAAAGAACTTCAATTGTACGCCAACT
TAAGACCATGTAACTTTGCATCCGACTCTCTTTTAGACTTATCTCCAATCAAGCCACAAT
TTGCTAAAGGTACTGACTTCGTTGTTGTCAGAGAATTAGTGGGAGGTATTTACTTTGGTA
AGAGAAAGGAAGACGATGGTGATGGTGTCGCTTGGGATAGTGAACAATACACCGTTCCAG
AAGTGCAAAGAATCACAAGAATGGCCGCTTTCATGGCCCTACAACATGAGCCACCATTGC
CTATTTGGTCCTTGGATAAAGCTAATGTTTTGGCCTCTTCAAGATTATGGAGAAAAACTG
TGGAGGAAACCATCAAGAACGAATTCCCTACATTGAAGGTTCAACATCAATTGATTGATT
CTGCCGCCATGATCCTAGTTAAGAACCCAACCCACCTAAATGGTATTATAATCACCAGCA
ACATGTTTGGTGATATCATCTCCGATGAAGCCTCCGTTATCCCAGGTTCCTTGGGTTTGT
TGCCATCTGCGTCCTTGGCCTCTTTGCCAGACAAGAACACCGCATTTGGTTTGTACGAAC
CATGCCACGGTTCTGCTCCAGATTTGCCAAAGAATAAGGTTGACCCTATCGCCACTATCT
TGTCTGCTGCAATGATGTTGAAATTGTCATTGAACTTGCCTGAAGAAGGTAAGGCCATTG
AAGATGCAGTTAAAAAGGTTTTGGATGCAGGTATCAGAACTGGTGATTTAGGTGGTTCCA
ACAGTACCACCGAAGTCGGTGATGCTGTCGCCGAAGAAGTTAAGAAAATCCTTGCTTAAA
AAGATTCTCTTTTTTTATGATATTTGTACATAAACTTTATAAATGAAATTCATAATAGAA
ACGACACGAAATTACAAAATGGAATATGTTCATAGGGTAGACGAAACTATATACGCAATC
TACATACATTTATCAAGAAGGAGAAAAAGGAGGATAGTAAAGGAATACAGGTAAGCAAAT
TGATACTAATGGCTCAACGTGATAAGGAAAAAGAATTGCACTTTAACATTAATATTGACA
AGGAGGAGGGCACCACACAAAAAGTTAGGTGTAACAGAAAATCATGAAACTACGATTCCT
AATTTGATATTGGAGGATTTTCTCTAAAAAAAAAAAAATACAACAAATAAAAAACACTCA
ATGACCTGACCATTTGATGGAGTTTAAGTCAATACCTTCTTGAACCATTTCCCATAATGG
TGAAAGTTCCCTCAAGAATTTTACTCTGTCAGAAACGGCCTTACGACGTAGTCGATATGG
TGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCA
ACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCT
GTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGACTGCAGCA
TTTCCAAACATTTTATTTCGCCACAACTTGTAGTTTTGTATTGACTCAAATACTTTCTCT
TGTTTTGTGCTTCTATTACAGAGATGGCTTTTTTCTTCAAGTACACTATTTCTAGACGCA
CTGCACTCGTCAAAAAAATGATTCAAGGTGAAAATAAAGGGGTGTTAGTGAATACTTATC
AAGAAGAGACATACATAATTGTACTGCTGTGCATTTTCCTACTTGACTTTCATGATTATG
AATAGTACAAACACAGTTGTATATATCAAAGTTAAGGGTAGGAGACCACAGGGATTCTTG
GATCCTCCCAAATTTGAGTGGAATGGAACAAAGGAGCGACAGCTTTGGACAATGGTATCA
AATTTGAATTATTCACAAGATCAAATAGATTGGCAGAATTTATCAAAAATATTTGAAACG
CCTGAATTTTTTCTTAAAAAGAGAACATATAAATTATTTGCTGAACACTTAGAGCTTTTA
CAACTACAATTGGAGAAAAAAAGAGATCTTGAGAAGTATTCAAATGATCAAGTGAATGAA
GGGATGTCTGATTTAATACATAAATATACCCCTACTTTACAAAATGATAATCTATTAAAT
GTATCCGCAAGCCCACTGACCACGGAAAGACAGGATTCTGAAGAAGTTGAGACAGAAGTA
ACAAATGAGGCGTTACAACATTTGCAAACTTCCAAAATTTTGAACATTCACAAGAAAACA
TCGGATAGTGAGAATAAGCCTAATGACAAATTGGATAAGGATGGTATTAATAAAGAAATG
GAGTGCGGTAGTTCAGATGACGATTTATCTTCGAGCTTAAGTGTTAGTAAATCTGCGTTG
GAAGAAGCGCTAATGGACAGATTGCAATTCTGATTTACATCTTTCACAAGCACAATCAAA
AAACCAACTGTCGGCCAAAAAGGCCCGGCGTTTTACTGTTGGTAAGTCCAGTACCCCGGA
ATAATCGATACAGATCTGCTCATCCTTTTTTATATCTCTATTCATAGTGAATAGCATTGA
ATTACCTTTACGATATTTCGTTATATTGGGGTTACAAGAGTGGTTGAAATAGGAGGCTTC
TGGAAATACCCAGTATCCAAAATATTCTCTGCTGTCTGAGGCTTCGCCCTCTTGCCATAA
ACCAAAAGCATGTCGACCTCGAGGGGGGGCCCGGTACCCAGCTTTTGTTCCCTTTAGTGA
GGGTTAATTCCGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTAT
CCGCTCACAATTCCACACAACATAGGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCC
TAATGAGTGAGGTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGA
AACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGT
ATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGG
CGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAAC
GCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCG
TTGCTGGCGTTTTTCCATAGGCTCGGCCCCCCTGACGAGCATCACAAAAATCGACGCTCA
AGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTCCCCCCTGGAAGC
TCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTC
CCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAG
GTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCC
TTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCA
GCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTG
AAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTG
AAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCT
GGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAA
GAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAA
GGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAA
TGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGC
TTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGA
CTGCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCA
ATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCC
GGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAAT
TGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCC
ATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGT
TCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGAAAAAAAGCGGTTAGCTCC
TTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATG
GCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGT
GAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCG
GCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGA
AAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATG
TAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGG
TGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGT
TGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTC
ATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACA
TTTCCCCGAAAAGTGCCACCTGGGTCCTTTTCATCACGTGCTATAAAAATAATTATAATT
TAAATTTTTTAATATAAATATATAAATTAAAAATAGAAAGTAAAAAAAGAAATTAAAGAA
AAAATAGTTTTTGTTTTCCGAAGATGTAAAAGACTCTAGGGGGATCGCCAACAAATACTA
CCTTTTATCTTGCTCTTCCTGCTCTCAGGTATTAATGCCGAATTGTTTCATCTTGTCTGT
GTAGAAGACCACACACGAAAATCCTGTGATTTTACATTTTACTTATCGTTAATCGAATGT
ATATCTATTTAATCTGCTTTTCTTGTCTAATAAATATATATGTAAAGTACGCTTTTTGTT
GAAATTTTTTAAACCTTTGTTTATTTTTTTTTCTTCATTCCGTAACTCTTCTACCTTCTT
TATTTACTTTCTAAAATCCAAATACAAAACATAAAAATAAATAAACACAGAGTAAATTCC
CAAATTATTCCATCATTAAAAGATACGAGGCGCGTGTAAGTTACAGGCAAGCGATCCGTC
CTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTT
TCGTC
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Konstrukcja: |
To construct rev3::LEU2 disruption cassette fragment of DNA containing LEU2 gene was PCR amplified using primers: 5' CGGCTGCAGTCGGTGATGACGGTGAAAAC 3' (PstI) and 5' ATTCCCGGGAAAAACTTGATTAGGGTGATGG 3' (SmaI) and pRS315 (LEU2) as a template. The resulting PCR product (2722 bp) was used to replace the CaURA3 marker in the pGJ100. Thus pGJ100 was PstI/SmaI digested and the fragment containing CaURA3 gene was replaced with PstI/SmaI digested fragment containing LEU2 gene. The resulting plasmid, named pGJ101, contains rev3::LEU2 dissruption cassette. All PCR products were confirmed by DNA sequencing. |
Plasmid properties/Comments: |
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Mapa plazmidu: |
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Markery selekcyjne: |
AmpR |
Stężenie antybiotyku: |
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Wielkość plazmidu: |
9545 bp |
Origin of replication/Incompatibility group: |
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Host range: |
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Inne elementy genetyczne: |
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Właściwości szczepu/komentarz: |
pGJ101 contain rev3::LEU2 disruption cassette |
Autor: |
Malgorzata Jaszczur |
Dostępność: |
restricted |
Patent: |
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Osoba deponująca: |
Malgorzata Jaszczur |
Instytucja/wydział: |
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Data zdeponowania: |
2009-03-21 17:53+01:00 |
Referencje: |
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