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Central Collection of Strains (IBB)

Base: Plasmid collection
Number in base: 2678
Plasmid name: pAMB11.41
Species: E. coli
Strain: DH5α
Genotype: F-(Φ80d lacZ ΔM15) recA1 endA1 gyrA96 thi-1 hsdR17(rk- mk+) supE44 relA1 deoR ΔlacZYA-argF) U196
Plasmid Sequence: unaviable
Construction: T7p– His6– repA-1; 293 nt fragment (RA3 coordinates 45441-45737) containing repA-1RA3 allele encodes RepAT32P cloned as the EcoRI-SalI fragment into pET28a without NdeI, BamHI sites and T7 tag
Plasmid properties/Comments:
Plasmid map:
Selectable Markers: kan
Antibiotic concetration:
Plasmid size: 5.7 kb
Origin of replication/Incompatibility group: oriMB1
Host range:
Other genetic elements:
Strain properties/Comments:
Author: Aleksandra Markowska
Availability: restricted
Patent:
Depositor: Aleksandra Markowska
Institution/Department: Zak?ad Biochemii Drobnoustrojów
Deposition date: 26.08.2014
References: