| Base: |
Plasmid collection |
| Number in base: |
269 |
| Plasmid name: |
p220 |
| Species: |
E. coli |
| Strain: |
DH5α |
| Genotype: |
supE44 Δ lacU169 (PHI 80 lacZ Δ M15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 |
| Plasmid Sequence: |
|
| Construction: |
Ω ORF of pDB101 amplified by PCR (positions 6512-6727) and inserted into pBT205 vector |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp, cam |
| Antibiotic concetration: |
|
| Plasmid size: |
about 11kb |
| Origin of replication/Incompatibility group: |
colEI |
| Host range: |
E.coli |
| Other genetic elements: |
|
| Strain properties/Comments: |
plasmid used for construction of YB886/220 strain |
| Author: |
I.Sitkiewicz |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
I. Sitkiewicz |
| Institution/Department: |
|
| Deposition date: |
06.08.2002 |
| References: |
I. Sitkiewicz, PhD thesis |