Base: |
Plasmid collection |
Number in base: |
294 |
Plasmid name: |
pBT205Δ |
Species: |
E. coli |
Strain: |
DH5α |
Genotype: |
F-(φ80 lacZΔM15) recA1endA1 gyrA96 thi1 hsdR17 (r-km-k) supE44 relA1 deoR Δlac(ZYA-argF)U169 |
Plasmid Sequence: |
|
Construction: |
plasmid pBT233 was digested by HincII and BbrPI, (positions 4692 and 5797 of pDB101, respectively) and a 1105bp fragment Δ promoter was cloned into pBT205 cleaved by
EcoRI, HindIII and blunted. |
Plasmid properties/Comments: |
|
Plasmid map: |
|
Selectable Markers: |
amp, cam |
Antibiotic concetration: |
|
Plasmid size: |
11 kb |
Origin of replication/Incompatibility group: |
colE1 |
Host range: |
|
Other genetic elements: |
|
Strain properties/Comments: |
plasmid used for integration of copS promoter into amyE locus of B. subtilis chromosome |
Author: |
Piotr Ceglowski |
Availability: |
restricted |
Patent: |
|
Depositor: |
I. Sitkiewicz |
Institution/Department: |
|
Deposition date: |
06.08.2002 |
References: |
I.Sitkiewicz PhD thesis |