| Base: |
Plasmid collection |
| Number in base: |
301 |
| Plasmid name: |
pBT205epsilon |
| Species: |
Escherichia coli |
| Strain: |
DH5α |
| Genotype: |
F-(f80 lacZΔM15) recA1endA1 gyrA96 thi1 hsdR17 (r-km-k) supE44 relA1 deoR Δlac(ZYA-argF)U169 |
| Plasmid Sequence: |
|
| Construction: |
Fragment (the epsilon promoter) was amplified in a PCR reaction (positions 6529-6915 of pDB101), HindIII digested and cloned into pBT205 vector digested by EcoRI, blunted by T4 polymerase and digested by HindIII |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp, cam |
| Antibiotic concetration: |
|
| Plasmid size: |
about 11kb |
| Origin of replication/Incompatibility group: |
|
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
|
| Author: |
Piotr Ceglowski |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
I. Sitkiewicz |
| Institution/Department: |
|
| Deposition date: |
06.08.2002 |
| References: |
I. Sitkiewicz PhD thesis |