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Central Collection of Strains (IBB)

Base: Plasmid collection
Number in base: 302
Plasmid name: pRP346-8cm
Species: Escherichia coli
Strain: DH5α
Genotype: F-(f80 lacZΔM15) recA1 endA1 gyrA96 thi1 hsdR17 (r-km-k) supE44 relA1 deoR Δlac(ZYA-argF)U169
Plasmid Sequence:
Construction: promoterless 1.3 kb fragment maplified by PCR amplified by PCR (positions 6595-7880 of pDB101) inserted into PstI/BamHI sites of pHP13. Fragment carries two point mutations in positions 7089 (A to T) and 7184 (G to A)
Plasmid properties/Comments:
Plasmid map:
Selectable Markers: ery, cam
Antibiotic concetration:
Plasmid size: 6,2kb
Origin of replication/Incompatibility group: colEI/pTA1060
Host range: E. coli/B. Subtilis
Other genetic elements:
Strain properties/Comments:
Author: R. Pankiewicz
Availability: restricted
Patent:
Depositor: I. Sitkiewicz
Institution/Department:
Deposition date: 06.08.2002
References: I. Sitkiewicz PhD thesis