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Central Collection of Strains (IBB)

Base: Plasmid collection
Number in base: 59
Plasmid name: pKG12
Species: E. coli
Strain: DH5α
Genotype: supE44, lacU169(FI 80lacZ Δ M15), hsdR17, recA1, endA1, gyrA96, thi-1, relA1;
Plasmid Sequence:
Construction: 2.2 kb BamHI insert carrying yeast erg20-2 gene tagged on 5` end with ProtA epitope from Staphyloccocus aureus under yeast NOP1promoter in pRS316 plasmid
Plasmid properties/Comments:
Plasmid map:
Selectable Markers: amp, URA3
Antibiotic concetration:
Plasmid size: 7080 bp
Origin of replication/Incompatibility group:
Host range:
Other genetic elements:
Strain properties/Comments:
Author: K. Grabinska
Availability: restricted
Patent:
Depositor: K. Grabinska
Institution/Department:
Deposition date: 1.03.2002
References: K. Grabinska, PhD thesis 2002