Baza: |
Plazmidów |
Nr w bazie: |
626 |
Nazwa plazmidu: |
pMLO36 |
Gatunek: |
Escherichia coli K12 |
Szczep: |
DJ125 (=C600 recA) |
Genotyp: |
supE44 hsdR17 thr-1 leuB6 lacY1 thi-1 tonA21 recA
|
Sekwencja plazmidu: |
available on request |
Konstrukcja: |
Spontaneous mutant of pMLO5 (pGB2ts-parS+)(isolated as 5/1) that is not dramatically destabilized in DJ125 lysogenized with lambda DKC296 (parA+parB+). Contains substitution of CCA between two arms of the large P1 parS palindrome by TGG (the sequence of the relevant fragment of parS in the mutant is: GTGAAATCGTGGCGATTTCACCTTGGATTTTAC)
|
Plasmid properties/Comments: |
|
Mapa plazmidu: |
|
Markery selekcyjne: |
spc, str |
Stężenie antybiotyku: |
|
Wielkość plazmidu: |
4.3 kb |
Origin of replication/Incompatibility group: |
pSC101 (ts)
|
Host range: |
Escherichia coli |
Inne elementy genetyczne: |
|
Właściwości szczepu/komentarz: |
Derivative of pMLO5 (pGB2ts-parS+) containing P1 parS site mutant that makes pMLO5 resistant to dramatic destabilization by ParB. This plasmid can not replicate at 42oC but can replicate at 30oC. |
Autor: |
Malgorzata Lobocka |
Dostępność: |
restricted |
Patent: |
|
Osoba deponująca: |
Malgorzata Lobocka |
Instytucja/wydział: |
|
Data zdeponowania: |
2006-07-02 23:10+01:00 |
Referencje: |
|