| Base: |
Plasmid collection |
| Number in base: |
88 |
| Plasmid name: |
pYEF1JA2His |
| Species: |
Escherichia coli |
| Strain: |
DH5α |
| Genotype: |
F-, f80dlacZDM15 Dlac(lac ZYA-argF)U169, deoR, hsdR17 (rk-,mk-), recA1, endA1, gyrA46, pho A, sup E44, thi-1, relA1 |
| Plasmid Sequence: |
|
| Construction: |
derivative of pYEF1, 2200 bp insert, coding for the yeast RNA helicase JA2 (YKL078W), with 6xHis epitop added at the C-end, cloned using NotI and EcoRI sites |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp, URA3 |
| Antibiotic concetration: |
|
| Plasmid size: |
9293 bp |
| Origin of replication/Incompatibility group: |
2 &mikro;m |
| Host range: |
E.coli, S. cerevisiae |
| Other genetic elements: |
|
| Strain properties/Comments: |
|
| Author: |
Boguszewska-Chachulska A.M. |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Iwona Rosa |
| Institution/Department: |
|
| Deposition date: |
29.03.2002 |
| References: |
|