| Base: |
Plasmid collection |
| Number in base: |
12 |
| Plasmid name: |
pGB2λ |
| Species: |
Escherichia coli |
| Strain: |
DH5α |
| Genotype: |
F- gyrA96 recA1 relA1 endA1 thi-1 hsdR17 (r-m+) glnV44deoR Δ(lacZYA-aroF)U169 [φ80dΔ(lacZ)M15 |
| Plasmid Sequence: |
|
| Construction: |
1,35kb EcoRI/BamHI fragment of pMY17-3 ligated to EcoRI/BamHI of pGB2 vector |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
Spc |
| Antibiotic concetration: |
|
| Plasmid size: |
5,35kb |
| Origin of replication/Incompatibility group: |
pSC101 |
| Host range: |
E. coli |
| Other genetic elements: |
λ phage promoters PR and PL, cI857, |
| Strain properties/Comments: |
|
| Author: |
Zielenkiewicz U. |
| Availability: |
restricted |
| Patent: |
- |
| Depositor: |
U. Zielenkiewicz |
| Institution/Department: |
|
| Deposition date: |
16.09.2002 |
| References: |
Zielenkiewicz U. Role of genes epsilon and zeta of plasmid pSM19035 in its stable maintenance in bacterial cells. 2001 Ph.D.thesis, IBB PAN |