| Base: |
Plasmid collection |
| Number in base: |
28 |
| Plasmid name: |
pX-ε |
| Species: |
Escherichia coli |
| Strain: |
DH5α |
| Genotype: |
F- gyrA96 recA1 relA1 endA1 thi-1 hsdR17 (r-m+) glnV44deoR Δ(lacZYA-aroF)U169 [φ80dΔ(lacZ)M15 |
| Plasmid Sequence: |
|
| Construction: |
317bp fragment SspI-BamHI from PCR product (containing epsilon gene of pSM19035 plasmid), inserted into BamHI-(SpeI) of pX vector |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
Amp(E.coli) cam (B.subtilis) |
| Antibiotic concetration: |
|
| Plasmid size: |
7,8kb |
| Origin of replication/Incompatibility group: |
ColE1 |
| Host range: |
E.coli, B.subtilis(integration) |
| Other genetic elements: |
5`-,3`-ends of amyE locus |
| Strain properties/Comments: |
- |
| Author: |
U.Zielenkiewicz |
| Availability: |
restricted |
| Patent: |
- |
| Depositor: |
U. Zielenkiewicz |
| Institution/Department: |
|
| Deposition date: |
16.09.2002 |
| References: |
Zielenkiewicz U. Role of genes epsilon and zeta of plasmid pSM19035 in its stable maintenance in bacterial cells. 2001 Ph.D. Thesis, IBB PAN |