| Base: |
Plasmid collection |
| Number in base: |
280 |
| Plasmid name: |
pGADΔ |
| Species: |
E. coli |
| Strain: |
DH5α |
| Genotype: |
supE44 Δ lacU169 (PHI 80 lacZ Δ M15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 |
| Plasmid Sequence: |
|
| Construction: |
plasmid encoding translational fusion of GAL4 activating domain and Δ of pDB101. Insert ligated with pGAD424 vector |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp, LEU |
| Antibiotic concetration: |
|
| Plasmid size: |
7,7 |
| Origin of replication/Incompatibility group: |
colEI, 2MICRO |
| Host range: |
E.coli/ S.cerevisiae |
| Other genetic elements: |
|
| Strain properties/Comments: |
bifunctional vector for yeast two hybrid system |
| Author: |
I.Sitkiewicz |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
I. Sitkiewicz |
| Institution/Department: |
|
| Deposition date: |
06.08.2002 |
| References: |
I. Sitkiewicz, PhD thesis |