| Base: |
Bacterial Strains |
| Number in base: |
2816 |
| Species: |
E. coli |
| Strain: |
BL21 Rosetta (DE3) |
| Genotype: |
F- ompT hsdSB (rB - mB -) gal dcm (DE3) pRARE (argU, argW, ileX, glyT, leuW, proL) (CmR) |
| Strain category: |
0 |
| Phage sensitivities: |
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| Isolant: |
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| Detailed description of the source: |
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| Date of isolation: |
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| Plasmid name: |
NUDIX1 P. ramorum |
| Plasmid map: |
 |
| Selectable Markers: |
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| Antibiotic Concentration: |
Amp, Cam |
| Construction: |
The 1020bp cDNA of NUDIX1 gene from P. ramorum (collection nr 2127) was cloned into pQE-80L expression vector using BamHI and PstI restriction sites. The 54 bp signal sequence from 5' end of the insert was removed. The BL21 Rosetta (DE3) cells were transformed by the construct. |
| Strain properties/Comments: |
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| Author: |
Olejnik Kamil |
| Availability: |
restricted |
| Patent: |
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| Depositor: |
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| Institution/Department: |
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| Deposition date: |
2008-04-07 11:17+01:00 |
| Site of storage: |
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| References: |
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