| Base: |
Plasmid collection |
| Number in base: |
299 |
| Plasmid name: |
pBT346-5 |
| Species: |
Escherichia coli |
| Strain: |
DH5α |
| Genotype: |
F-(f80 lacZΔM15) recA1endA1 gyrA96 thi1 hsdR17 (r-km-k) supE44 relA1 deoR Δlac(ZYA-argF)U169 |
| Plasmid Sequence: |
|
| Construction: |
mutated Ω gene (6th codon exchanged into the stop) with natural promoter inserted into pHP13 vector |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
ery, cam |
| Antibiotic concetration: |
|
| Plasmid size: |
about 5,5kb |
| Origin of replication/Incompatibility group: |
colE1, pTA1060 |
| Host range: |
bifunctional plasmid, E. coli/Bacillus sp. |
| Other genetic elements: |
|
| Strain properties/Comments: |
for detailed scheme of the construction see I.Sitkiewicz PhD thesis |
| Author: |
Piotr Ceglowski |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
I. Sitkiewicz |
| Institution/Department: |
|
| Deposition date: |
06.08.2002 |
| References: |
de la Hoz et al., 2000 PNAS 18;97(2):728-33 |