| Base: |
Plasmid collection |
| Number in base: |
300 |
| Plasmid name: |
pRC1 |
| Species: |
Bacillus subtilis |
| Strain: |
YB886 |
| Genotype: |
amyE metB5 trpC2 xin-1 attSPbβ |
| Plasmid Sequence: |
|
| Construction: |
pDB101 derivative, carrying copS with its promoter, repS and ery genes. Blunted by T4 polymerase fragment EcoRI-FspI (positions 16738 and 2546 of pDB101) was ligated with HindIII-HindIII (blunted) fragment encompassing erm1 and erm2 genes (7824–9622) |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
ery |
| Antibiotic concetration: |
|
| Plasmid size: |
6831bp |
| Origin of replication/Incompatibility group: |
inc18 |
| Host range: |
Gram positive bacteria |
| Other genetic elements: |
|
| Strain properties/Comments: |
pSm19035 derivative |
| Author: |
I. Sitkiewicz |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
I. Sitkiewicz |
| Institution/Department: |
|
| Deposition date: |
06.08.2002 |
| References: |
I. Sitkiewicz, PhD thesis |