| Base: |
Plasmid collection |
| Number in base: |
302 |
| Plasmid name: |
pRP346-8cm |
| Species: |
Escherichia coli |
| Strain: |
DH5α |
| Genotype: |
F-(f80 lacZΔM15) recA1 endA1 gyrA96 thi1 hsdR17 (r-km-k) supE44 relA1 deoR Δlac(ZYA-argF)U169 |
| Plasmid Sequence: |
|
| Construction: |
promoterless 1.3 kb fragment maplified by PCR amplified by PCR (positions 6595-7880 of pDB101) inserted into PstI/BamHI sites of pHP13. Fragment carries two point mutations in positions 7089 (A to T) and 7184 (G to A) |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
ery, cam |
| Antibiotic concetration: |
|
| Plasmid size: |
6,2kb |
| Origin of replication/Incompatibility group: |
colEI/pTA1060 |
| Host range: |
E. coli/B. Subtilis |
| Other genetic elements: |
|
| Strain properties/Comments: |
|
| Author: |
R. Pankiewicz |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
I. Sitkiewicz |
| Institution/Department: |
|
| Deposition date: |
06.08.2002 |
| References: |
I. Sitkiewicz PhD thesis |