| Base: |
Plasmid collection |
| Number in base: |
36 |
| Plasmid name: |
pBT346-8m |
| Species: |
Escherichia coli |
| Strain: |
DH5α |
| Genotype: |
F-(φ80dlacZΔM15) recA1 endA1 gyrA96 thi-1 hsdR17(rk-mk+)supE44relA1deoR Δ(lacZYA-argF)U169 |
| Plasmid Sequence: |
|
| Construction: |
1,3kb PCR product corresponding to epsilon -zeta genes of pSM19035 plasmid (S. pyogenes) inserted into PstI-BamHI sites of pHP13 vector. Zeta gene contains two point mutations (A7089T, G7184A) and is inactive. |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
ery cam |
| Antibiotic concetration: |
|
| Plasmid size: |
6,2kb |
| Origin of replication/Incompatibility group: |
pBR322, pTA1060 |
| Host range: |
E.coli, B.subtilis |
| Other genetic elements: |
|
| Strain properties/Comments: |
|
| Author: |
R.Pankiewicz |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
U. Zielenkiewicz |
| Institution/Department: |
|
| Deposition date: |
16.09.2002 |
| References: |
|