| Base: |
Yeast strains |
| Institution Code: |
IBBPAS |
| Collection Code: |
ColIBB |
| Number in base: |
4008 |
| Species: |
Saccharomyces cerevisiae |
| Strain: |
SC48 |
| Genotype: |
MAT α his3Δ1 leu2Δ0 lys2Δ0 MET15 ura3Δ0 dpb2::kanMX4 [pMJ113(pRS313 HIS3 dpb2-113)]
|
| Plasmid: |
pMJ113 = pRS313 HIS3 dpb2-113, old pasmid name pGJ9 |
| Plasmid map: |
 |
| Selectable Markers: |
Leu Lys Ura kanMX4(G418) Amp |
| Antibiotic concentration: |
|
| Construction: |
Derivative of SC11 haploid strain (no.2893D); SC11 was transformed with pMJ113; transformants were picked twice throught 5-FOA containing plates to lose pMJDPB2 (URA3) plasmid
|
| Strain properties/Comments: |
strain grows well at 23oC, not growing at 37oC;
pMJ113 allele encodes mutated variant of Dpb2p protein with Lys171Glu Ser182Pro Leu284Pro substitutions; strain exhibits strong mutator phenotype
|
| Author: |
Malgorzata Jaszczur |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Malgorzata Jaszczur |
| Deposition date: |
2009-03-04 18:03+01:00 |
| References: |
Jaszczur et al. 2009 Mutation Research |