| Base: |
Yeast strains |
| Institution Code: |
IBBPAS |
| Collection Code: |
ColIBB |
| Number in base: |
4011 |
| Species: |
Saccharomyces cerevisiae |
| Strain: |
ΔI(-2)I-7B-YUNI300 pol2-4 |
| Genotype: |
MATa trp1-289 his7-2 leu2-Δ::kanMX4 ura3-Δ ade2-1 lys2-ΔGG2899-2900 CAN1 pol2-4 |
| Plasmid: |
|
| Plasmid map: |
 |
| Selectable Markers: |
trp, leu, lys, his, ura, ade, kanMX4(G418), CAN1 |
| Antibiotic concentration: |
|
| Construction: |
|
| Strain properties/Comments: |
strain grows well at 23-37oC; strain express mutator phenotype due to the mutation in the Polε proofreading activity; mutation frequencies can be measured at different genetic loci; trp1-289,ade2-1 allow to measure base substitution; his7-2 lys2-[ |
| Author: |
Pavlov Y. |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Malgorzata Jaszczur |
| Deposition date: |
2009-03-04 18:03+01:00 |
| References: |
Jaszczur et al. Genetics 178 (2008) |