| Base: |
Yeast strains |
| Institution Code: |
IBBPAS |
| Collection Code: |
ColIBB |
| Number in base: |
4053 |
| Species: |
Saccharomyces cerevisiae |
| Strain: |
SC280 |
| Genotype: |
MAT α his3Δ1 leu2Δ0 lys2Δ0 MET15 ura3Δ0 dpb2::kanMX4 rev3::CaURA3[pMJ103(pRS313 dpb2-103 HIS3 )]
|
| Plasmid: |
pMJ103 = pRS313 dpb2-103 HIS3 |
| Plasmid map: |
 |
| Selectable Markers: |
Leu Lys kanMX4 |
| Antibiotic concentration: |
|
| Construction: |
Derivative of 4001D (SC23); transformed with rev3::CaURA3 disruption cassette, integration of rev3::CaURA3 cassette to the chromosome leads to the deletion of entire REV3 ORF; disruption confirmed with PCR |
| Strain properties/Comments: |
strain express mutator phenotype when comparing with DPB2rev3 strain; rev3::CaURA3 mutations in dpb2-103 strain decreases rate of mutagenesis |
| Author: |
Malgorzata Jaszczur |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Malgorzata Jaszczur |
| Deposition date: |
2009-03-04 18:03+01:00 |
| References: |
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