| Base: |
Yeast strains |
| Institution Code: |
IBBPAS |
| Collection Code: |
ColIBB |
| Number in base: |
4062 |
| Species: |
Saccharomyces cerevisiae |
| Strain: |
SC383 |
| Genotype: |
MATa trp1-289 his7-2 leu2-Δ::kanMX4 ura3-Δ ade2-1 lys2-ΔGG2899-2900 CAN1 dpb2::pKF117dpb2-103(CaURA3, HIS3)rev3::LEU2
|
| Plasmid: |
|
| Plasmid map: |
 |
| Selectable Markers: |
trp, lys, his, ade, kanMX4(G418), CAN1 |
| Antibiotic concentration: |
|
| Construction: |
derivative of no. 4045 (SC152); SC152 was transformed with rev3::LEU2 disruption cassette; inetgration of rev3::LEU2 cassette leads to the deletion of the entire REV3 ORF; disruption confirmed by PCR
|
| Strain properties/Comments: |
strain grows well at 23oC, slowly at 30oC and does not grow at 37oC; strain express mutator phenotype; mutation frequencies can be measured at different genetic loci; trp1-289,ade2-1 allow to measure base substitution; his7-2 lys2-ΔGG2899-2900 allow |
| Author: |
Malgorzata Jaszczur |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Malgorzata Jaszczur |
| Deposition date: |
2009-03-04 18:03+01:00 |
| References: |
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