| Base: |
Plasmid collection |
| Number in base: |
45 |
| Plasmid name: |
pGAD424-Δ3ε |
| Species: |
Escherichia coli |
| Strain: |
DH5α |
| Genotype: |
F-(f80dlacZΔM15) recA1 endA1 gyrA96 thi-1 hsdR17(rk-mk+)supE44relA1deoR Δ(lacZYA-argF)U169 |
| Plasmid Sequence: |
|
| Construction: |
255bp PCR product corresponding to 5C-terminal amino acids deletion of epsilon gene (of pSM19035 plasmid from S.pyogenes) inserted into EcoRI-BamHI sites of pGAD424 vector (two-hybrid system) |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp |
| Antibiotic concetration: |
|
| Plasmid size: |
6,91kb |
| Origin of replication/Incompatibility group: |
|
| Host range: |
E. coli; S. cerevisiae |
| Other genetic elements: |
|
| Strain properties/Comments: |
|
| Author: |
U.Zielenkiewicz |
| Availability: |
restricted |
| Patent: |
- |
| Depositor: |
U. Zielenkiewicz |
| Institution/Department: |
|
| Deposition date: |
16.09.2002 |
| References: |
Zielenkiewicz, U. Ph.D. thesis: Role of genes epsilon and zeta of plasmid pSM19035 in its stable maintenance in bacterial cells. Warszawa, 2001. |