| Base: |
Plasmid collection |
| Number in base: |
524 |
| Plasmid name: |
λ-P1:5R parPO parA parB::kan parS |
| Species: |
E.coli |
| Strain: |
C600 (EC5171) |
| Genotype: |
F- supE44 hsdR17 thi-1 thr-1 leuB6 lacY1 tonA21 |
| Plasmid Sequence: |
|
| Construction: |
The chimera obtained from λ-P1:5R-3 depleted of the att and int genes of λ, contains the P1 replication and partition region. The insertion of a kan cassette disrupts the P1 par operon inside parB at the PvuI site |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
kan |
| Antibiotic concetration: |
|
| Plasmid size: |
ca. 45 kb |
| Origin of replication/Incompatibility group: |
as plasmid–replication from P1 origin,as phage–from ori λ |
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
It carries the λ cI857; this repressor cI is temperature sensitive |
| Author: |
Barbara Funnell |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
O.Juszczuk |
| Institution/Department: |
|
| Deposition date: |
2004-05-06 |
| References: |
Funnell, B.E. (1988). J. Bacteriol. 170: 954-960. |