| Base: |
Plasmid collection |
| Number in base: |
542 |
| Plasmid name: |
pOBK27 |
| Species: |
E.coli |
| Strain: |
MC1061 recA- (EC5404) |
| Genotype: |
recA1 endA1 relA1 thi-1 gyrA-96 hsdR17 Δ lacU169 (φ80 lacZ Δ M15) supE44 |
| Plasmid Sequence: |
|
| Construction: |
isogenic parB- control for pOBK16 carrying tryptophane promoter, 4.2-kb fragment of pOBK16 depleted of parB was obtained after digestion with PflMI and partial digestion with EcoRI, then ends were repaired and religated |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
Spc/Str |
| Antibiotic concetration: |
|
| Plasmid size: |
ca. 4.2 kb |
| Origin of replication/Incompatibility group: |
ori pSC101 |
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
|
| Author: |
Olga Juszczuk |
| Availability: |
non restricted |
| Patent: |
|
| Depositor: |
O.Juszczuk |
| Institution/Department: |
|
| Deposition date: |
2004-05-06 |
| References: |
carries repAts gene for the temperature sensitive replication protein, the concentration of spc depends on quality of antibiotic (20-40 ug/ml in LB)), orientation of Ptrp towards repAts |