| Base: |
Plasmid collection |
| Number in base: |
543 |
| Plasmid name: |
pOBK26 |
| Species: |
E.coli |
| Strain: |
MC1061 recA- (EC5406) |
| Genotype: |
recA1 endA1 relA1 thi-1 gyrA-96 hsdR17 Δ lacU169 (φ80 lacZ Δ M15) supE44 |
| Plasmid Sequence: |
|
| Construction: |
pOBK16-derivative containing parB-gfp under control of tryptophane promoter-operator region, 1.86 kb EcoRI fragment of pOBK3 containing trpPO parB-gfp was ligated with 4.1 kb EcoRI fragment of pOBK16 |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
Spc/Str |
| Antibiotic concetration: |
|
| Plasmid size: |
ca. 6 kb |
| Origin of replication/Incompatibility group: |
ori pSC101 |
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
plasmid carries repAts gene for the temperature sensitive replication protein, the concentration of spc depends on quality of antibiotic (20-40 ug/ml in LB), orientation of parB-gfp towards repAts as in pOBK16 |
| Author: |
Olga Juszczuk |
| Availability: |
non available |
| Patent: |
|
| Depositor: |
O.Juszczuk |
| Institution/Department: |
|
| Deposition date: |
2004-05-06 |
| References: |
|