| Base: |
Plasmid collection |
| Number in base: |
547 |
| Plasmid name: |
λ-P1:5R kan Δ(parPO parAB) parS |
| Species: |
E.coli |
| Strain: |
DH5 Δ lac (EC5419) |
| Genotype: |
recA1 endA1 relA1 thi-1 gyrA-96 hsdR17 Δ lac supE44 |
| Plasmid Sequence: |
|
| Construction: |
The l-P1:5R kan Δ(parPOparAB) parS was obtained by homologous recombination between the recipient l-P1:5R kan parPOparABS plasmid and linear HindIII-NruI fragment of pOBK32. |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
kan |
| Antibiotic concetration: |
|
| Plasmid size: |
ca. 45 kb |
| Origin of replication/Incompatibility group: |
as plasmid – replication from P1 origin, as phage – from λ ori |
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
It carries the λ cI857; this repressor cI is temperature sensitive |
| Author: |
Olga Juszczuk |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
O.Juszczuk |
| Institution/Department: |
|
| Deposition date: |
2004-05-06 |
| References: |
|