| Base: |
Plasmid collection |
| Number in base: |
622 |
| Plasmid name: |
pMLO19 |
| Species: |
Escherichia coli K12 |
| Strain: |
DJ125 (=C600 recA); EC16825 |
| Genotype: |
supE44 hsdR17 thr-1 leuB6 lacY1 thi-1 tonA21 recA
|
| Plasmid Sequence: |
available on request
|
| Construction: |
Ligation of HindIII-BamHI fragment of pGB2ts with the large HindIII-BamH1 fragment of pBR322
|
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
spc; str; amp |
| Antibiotic concetration: |
|
| Plasmid size: |
~4 kb |
| Origin of replication/Incompatibility group: |
pSC101; pMB1
|
| Host range: |
Escherichia coli |
| Other genetic elements: |
|
| Strain properties/Comments: |
This plasmid is a hybrid of pBR322 and pGB2ts. At elevated temperature (42oC) it can replicate only as a high-copy number plasmid from the pMB1 origin of replication. |
| Author: |
Malgorzata Lobocka |
| Availability: |
non restricted |
| Patent: |
|
| Depositor: |
Malgorzata Lobocka |
| Institution/Department: |
|
| Deposition date: |
2006-07-02 12:09+01:00 |
| References: |
|