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Central Collection of Strains (IBB)

Base: Plasmid collection
Number in base: 798
Plasmid name: pADS78
Species: Escherichia coli K12
Strain: EC17243
Genotype: DH5α [recA1 endA1 relA1 thi-1 gyrA-96 hsdR17 ΔlacU169 (φ80 lacZ ΔM15) supE44]
Plasmid Sequence: .
Construction: pBEF116 derivative containing four copies of T1 transcription terminator of E. coli rrnB operon inserted in front of P1 parOP-parAparB operon; 844 bp MluI-SmaI fragment of pRS552 repaired at MluI end and cloned in ClaI-cleaved and end-repaired pBEF116
Plasmid properties/Comments:
Plasmid map:
Selectable Markers: amp
Antibiotic concetration:
Plasmid size: 7 kb
Origin of replication/Incompatibility group: pMB1
Host range:
Other genetic elements:
Strain properties/Comments:
Author: Aneta Dobruk-Serkowska
Availability: restricted
Patent:
Depositor: Aneta Dobr
Institution/Department:
Deposition date: 2004-10-21 10:03+01:00
References: Aneta Dobruk-Serkowska; Ph.D. thesis Warsaw 2004