| Base: |
Plasmid collection |
| Number in base: |
858 |
| Plasmid name: |
pACE2 |
| Species: |
Escherichia coli K12 |
| Strain: |
DJ125 |
| Genotype: |
supE44, hsdR, thi-1, trh-1, leuB6, lacY1, tonA21, recA56
|
| Plasmid Sequence: |
. |
| Construction: |
Insertion of SmaI-SphI fragment of pUCP7/679 containing the lyz in place of XhoI-SphI fragment of pACE1, repaired at XhoI end
|
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp |
| Antibiotic concetration: |
|
| Plasmid size: |
5114 |
| Origin of replication/Incompatibility group: |
pMB1
|
| Host range: |
E. coli |
| Other genetic elements: |
|
| Strain properties/Comments: |
This plasmid carries P1 lyz, lydD, lydE genes operon and the promoter-operator region of trp operon of Serratia marcescens. The lyz, lydD and lydE genes are not expressed due to the presence of long non coding fragment of DNA between the promotor and thes |
| Author: |
Agata Cena |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Agata Cena |
| Institution/Department: |
|
| Deposition date: |
2005-02-22 22:26+01:00 |
| References: |
A. Cena. Praca Magisterska, IBB, Warszawa, 2004 |