| Base: |
Plasmid collection |
| Number in base: |
86 |
| Plasmid name: |
pYEF1HCVhel |
| Species: |
Escherichia coli |
| Strain: |
DH5α |
| Genotype: |
F-, f80dlacZDM15 Dlac(lac ZYA-argF)U169, deoR, hsdR17 (rk-,mk-), recA1, endA1, gyrA46, pho A, sup E44, thi-1, relA1 |
| Plasmid Sequence: |
|
| Construction: |
derivative of the pYEF1 plasmid; 1500 bp insert, coding for the RNA helicase of the HCV virus, was cloned by the homologous recombination with the vector digested with NotI and EcoRI |
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
amp,URA3 |
| Antibiotic concetration: |
|
| Plasmid size: |
8509 bp |
| Origin of replication/Incompatibility group: |
2 &mikro;m |
| Host range: |
E.coli, S.cerevisiae |
| Other genetic elements: |
|
| Strain properties/Comments: |
|
| Author: |
Boguszewska-Chachulska A.M. |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Iwona Rosa |
| Institution/Department: |
|
| Deposition date: |
29.03.2002 |
| References: |
|