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Central Collection of Strains (IBB)

Base: Plasmid collection
Number in base: 900
Plasmid name: pSGUM2.4
Species: Eschericha coli
Strain: DH5α
Genotype: F-(Φ80 lacZΔM15) recA1endA1 gurA96 thil hsdR17 (r-K m-k) supE44 relA1 deoR Δlac (ZYA-argF)U169
Plasmid Sequence: N/A
Construction: Blunted DNA fragment of p1658/97 cloned into SmaI of the pSGUM2 vector.
Plasmid properties/Comments:
Plasmid map:
Selectable Markers: amp, cam
Antibiotic concetration:
Plasmid size: 3,7kb (vector) + more or less 1500bp (insert)
Origin of replication/Incompatibility group:
Host range: Enterobacteria
Other genetic elements:
Strain properties/Comments: Coordinates of the cloned DNA fragment: from about 3120 to 4620 according to p1658/97 annotation submitted in GeneBank under acc no AF550679. Insert contains traY and traA genes.The pSGUM2 it is a derivative of pUC13 (see reference:P. Fort, J. Errington
Author: Maksymilian Zienkiewicz
Availability: non restricted
Patent:
Depositor: Maksymilian Zienkiewicz
Institution/Department:
Deposition date: 2004-10-25 10:37+01:00
References: