|
2698 |
EC8419 EC8420 |
Escherichia coli |
amp spc str tet |
transduction of the strain EC6800, EC6801 with P1 phage grown on the strain mutL::Tn10 selection on medium containing tetracycline |
non restricted |
M. Banach - Or?owska |
M. Banach - Orłowska |
Recomended concentration of amp - 0,04mg/ml |
|
|
2699 |
EC8421 EC8422 |
Escherichia coli |
amp spc str tet |
transduction of the strain EC6802, EC6803 with P1 phage grown on the strain mutL::Tn10 selection on medium containing tetracycline |
non restricted |
M. Banach - Or?owska |
M. Banach - Orłowska |
Recomended concentration of amp - 0,04mg/ml |
|
|
2700 |
EC8423 EC8424 |
Escherichia coli |
amp spc str tet |
transduction of the strain EC7243, EC7244 with P1 phage grown on the strain mutL::Tn10 selection on medium containing tetracycline |
non restricted |
M. Banach - Or?owska |
M. Banach - Orłowska |
Recomended concentration of amp - 0,04mg/ml |
|
|
2701 |
EC8425 EC8426 |
Escherichia coli |
amp spc str tet |
transduction of the strain EC7246, EC7247 with P1 phage grown on the strain mutL::Tn10 selection on medium containing tetracycline |
non restricted |
M. Banach - Or?owska |
M. Banach - Orłowska |
Recomended concentration of amp - 0,04mg/ml |
|
|
2702 |
EC8447 EC8448 |
Escherichia coli |
amp kan |
transduction of the strain EC8416, EC8417 with P1 phage grown on the strain YG7207 (dinB::kan)selection on medium containing kanamycin |
non restricted |
M. Banach - Or?owska |
M. Banach - Orłowska |
Recomended concentration of amp - 0,04mg/ml |
|
|
2703 |
EC8450 EC8451 |
Escherichia coli |
amp spc str |
transduction of the strain EC8416, EC8417 with P1 phage grown on the strain NR11501 (polB::spc, str)selection on medium containing spectinomycin |
non restricted |
M. Banach - Or?owska |
M. Banach - Orłowska |
Recomended concentration of amp - 0,04mg/ml |
|
|
2704 |
EC8453 EC8454 |
Escherichia coli |
amp tet |
transduction of the strain EC8416, EC8417 with P1 phage grown on the strain CS115 (dnaE486-zae502::Tn10)selection on medium containing tetracycline |
non restricted |
M. Banach - Or?owska |
M. Banach - Orłowska |
Recomended concentration of amp - 0,04mg/ml
TS-42 C degrees |
|
|
2705 |
EC8456 EC8457 |
Escherichia coli |
amp kan tet |
transduction of the strain EC8447 EC83448 with P1 phage grown on the strain CS115 (dnaE486-zae502::Tn10) selection on the medium containing tetracycline
|
non restricted |
M. Banach - Or?owska |
M. Banach - Orłowska |
Recomended concentration of amp - 0,04mg/ml
TS- 42C degrees |
|
|
2706 |
EC8459 EC8460 |
Escherichia coli |
amp spc tet |
transduction of the strain EC8450 EC83451 with P1 phage grown on the strain CS115 (dnaE486-zae502::Tn10) selection on the medium containing tetracycline
|
non restricted |
M. Banach - Or?owska |
M. Banach - Orłowska |
Recomended concentration of amp - 0,04mg/ml
TS- 42C degrees |
|
|
2707 |
EC8473 EC8474 |
Escherichia coli |
amp spc tet kan spc |
transduction of the strain EC8149 EC8150 with P1 phage grown on the strain YG7207 (dinB::kan) selection on the medium containing kanamycin |
non restricted |
M. Banach - Or?owska |
M. Banach - Orłowska |
Recomended concentration of amp - 0,04mg/ml
TS- 42C degrees |
|
|
2708 |
EC8476 EC8477 |
Escherichia coli |
amp spc tet kan |
transduction of the strain EC8459 EC8460 with P1 phage grown on the strain YG7207 (dinB::kan) selection on the medium containing kanamycin |
non restricted |
M. Banach - Or?owska |
M. Banach - Orłowska |
Recomended concentration of amp - 0,04mg/ml
TS- 42C degrees |
|
|
2709 |
EC8498 EC8499 |
Escherichia coli |
amp tet kan |
transduction of the strain EC6949 EC6950 with P1 phage grown on the strain YG7207 (dinB::kan) selection on medium containing kanamycin |
non restricted |
M. Banach - Or?owska |
M. Banach - Orłowska |
Recomended concentration of amp - 0,04mg/ml
|
|
|
2710 |
EC8501 |
Escherichia coli |
amp tet cam |
transduction of the strain EC5583 with P1 phage grown on the strain EC3020 (ΔumuDC::cat) selection on medium containing chloramphenicol |
non restricted |
M. Banach - Or?owska |
M. Banach - Orłowska |
Recomended concentration of amp - 0,04mg/ml,
for recA730 mutants minimal plates recomended
|
|
|
2711 |
EC8502 EC8503 |
Escherichia coli |
amp cam |
transduction of the strain EC5584 EC5585 with P1 phage grown on the strain EC3020 (ΔumuDC::cat) selection on medium containing chloramphenicol |
non restricted |
M. Banach - Or?owska |
M. Banach - Orłowska |
Recomended concentration of amp - 0,04mg/ml,
for recA730 mutants minimal pletes recomended
|
|
|
2712 |
EC8504 |
Escherichia coli |
amp tet cam |
transduction of the strain EC8427 with P1 phage grown on the strain EC3020 (ΔumuDC::cat) selection on medium containing chloramphenicol |
non restricted |
M. Banach - Or?owska |
M. Banach - Orłowska |
Recomended concentration of amp - 0,04mg/ml,
for recA730 mutants minimal pletes recomended
|
|
|
2713 |
EC8505 |
Escherichia coli |
amp tet cam |
transduction of the strain EC8430 with P1 phage grown on the strain EC3020 (ΔumuDC::cat) selection on medium containing chloramphenicol |
non restricted |
M. Banach - Or?owska |
M. Banach - Orłowska |
Recomended concentration of amp - 0,04mg/ml,
for recA730 mutants minimal plates recomended
|
|
|
2714 |
EC8506 |
Escherichia coli |
amp kan cam |
transduction of the strain EC8501 with P1 phage grown on the strain YG7202 (dinB::kan) selection on medium containing kanamycin |
non restricted |
M. Banach - Or?owska |
M. Banach - Orłowska |
Recomended concentration of amp - 0,04mg/ml,
for recA730 mutants minimal plates recomended
|
|
|
2715 |
EC8507 EC8508 |
Escherichia coli |
amp kan cam |
transduction of the strain EC8502 EC8503 with P1 phage grown on the strain YG7202 (dinB::kan) selection on medium containing kanamycin |
non restricted |
M. Banach - Or?owska |
M. Banach - Orłowska |
Recomended concentration of amp - 0,04mg/ml,
for recA730 mutants minimal plates recomended
|
|
|
2716 |
EC8509 |
Escherichia coli |
amp kan cam tet |
transduction of the strain EC8504 with P1 phage grown on the strain YG7202 (dinB::kan) selection on medium containing kanamycin |
non restricted |
M. Banach - Or?owska |
M. Banach - Orłowska |
Recomended concentration of amp - 0,04mg/ml,
for recA730 mutants minimal plates recomended
|
|
|
2717 |
EC8510 EC8511 |
Escherichia coli |
amp kan cam tet |
transduction of the strain EC8505 with P1 phage grown on the strain YG7202 (dinB::kan) selection on medium containing kanamycin |
non restricted |
M. Banach - Or?owska |
M. Banach - Orłowska |
Recomended concentration of amp - 0,04mg/ml,
for recA730 mutants minimal plates recomended
|
|
|
2718 |
EC8672 |
Escherichia coli |
amp kan met |
transduction of the strain EC5390 with P1 phage grown on the strain YG7202 (dinB::kan) selection on medium containing kanamycin |
non restricted |
M. Banach - Or?owska |
M. Banach - Orłowska |
Recomended concentration of amp - 0,04mg/ml,
for recA730 mutants minimal plates recomended
|
|
|
2719 |
EC8673 |
Escherichia coli |
amp kan met |
transduction of the strain EC5391 with P1 phage grown on the strain YG7202 (dinB::kan) selection on medium containing kanamycin |
non restricted |
M. Banach - Or?owska |
M. Banach - Orłowska |
Recomended concentration of amp - 0,04mg/ml,
for recA730 mutants minimal plates recomended
|
|
|
2720 |
EC8676 EC8677 |
Escherichia coli |
amp kan pro thi |
transduction of the strain EC4206 EC4207 with P1 phage grown on the strain YG7202 (dinB::kan) selection on medium containing kanamycin |
non restricted |
M. Banach - Or?owska |
M. Banach - Orłowska |
Recomended concentration of amp - 0,04mg/ml,
for recA730 mutants minimal plates recomended
|
|
|
2721 |
EC8678 EC8679 |
Escherichia coli |
amp kan pro thi |
transduction of the strain EC4209 EC4210 with P1 phage grown on the strain YG7202 (dinB::kan) selection on medium containing kanamycin |
non restricted |
M. Banach - Or?owska |
M. Banach - Orłowska |
Recomended concentration of amp - 0,04mg/ml,
for recA730 mutants minimal plates recomended
|
|
|
2722 |
DE1872 |
Escherichia coli |
|
|
non available |
Koch, strain obtained from R. Woodgate |
Magdalena Banach-Orlowska |
|
Koch et al. MGG (1992)233:443-448 |
|
2723 |
DE1851 |
Escherichia coli |
|
|
non available |
Koch, strain obtained from R. Woodgate |
Magdalena Banach-Orlowska |
|
Koch et al. MGG (1992)233:443-448 |
|
2724 |
DE1868 |
Escherichia coli |
|
|
non available |
Koch, strain obtained from R. Woodgate |
Magdalena Banach-Orlowska |
|
Koch et al. MGG (1992)233:443-448 |
|
2800 |
BL21 Rosetta (DE3) |
E. coli |
Amp, Cam |
The wild type AtNUDT7 (construct 1791 P IBB Strain Colection)gene in pQE80L vector was mutated in position 171 TTC → GAC (Phe 171 to Ala). The first methionine ATG codon was removed from... |
restricted |
Olejnik Kamil |
Olejnik Kamil |
|
|
|
2801 |
BL21 Rosetta (DE3) |
E. coli |
Amp, Cam |
The wild type AtNUDT7 (construct 1791 P IBB Strain Colection)gene in pQE80L vector was mutated in position V168 GTA → GCA (Val 168 to Ala). The first methionine ATG codon was removed fro... |
restricted |
Olejnik kamil |
Olejnik kamil |
|
|
|
2802 |
BL21 Rosetta (DE3) |
E. coli |
Amp, Cam |
The wild type AtNUDT7 (construct 1791 P IBB Strain Colection)gene in pQE80L vector was mutated in positions 168 GTA → GCA and 171 TTC → GAC (Phe 171 to Ala, Val 168 to Ala). The fi... |
restricted |
Olejnik Kamil |
Olejnik Kamil |
|
|
|
2803 |
BL21 Rosetta (DE3) |
E. coli |
Amp, Cam |
The wild type AtNUDT7 (construct 1791 P IBB Strain Colection)gene in pQE80L vector was mutated in position 171 TTC → GCC (Phe 171 to Ala). The first methionine ATG codon was removed from... |
restricted |
Olejnik Kamil |
Olejnik Kamil |
|
|
|
2804 |
BL21 Rosetta (DE3) |
E. coli |
Amp, Cam |
The wild type AtNUDT7 (construct 1791 P IBB Strain Colection)gene in pQE80L vector was mutated in position 168 GTA → GCA and 171 TTC → GCC
(Val 168 to Ala, Phe 171 to Ala). The fi... |
restricted |
Olejnik Kamil |
Olejnik Kamil |
|
|
|
2805 |
BL21 Rosetta (DE3) |
E. coli |
Amp, Cam |
The wild type AtNUDT7 (construct 1791 P IBB Strain Colection)gene in pQE80L vector was mutated in position 210 TGG → GCG(Trp 210 to Ala). The first methionine ATG codon was removed from ... |
restricted |
Olejnik Kamil |
Olejnik Kamil |
|
|
|
2806 |
BL21 Rosetta (DE3) |
E. coli |
Amp, Cam |
The wild type AtNUDT7 (construct 1791 P IBB Strain Colection)gene in pQE80L vector was mutated in position 45 TGG → GCG (Trp 45 to Ala). The first methionine ATG codon was removed from t... |
restricted |
Olejnik Kamil |
Olejnik Kamil |
|
|
|
2807 |
BL21 Rosetta (DE3) |
E. coli |
Amp, Cam |
The wild type AtNUDT7 (construct 1791 P IBB Strain Colection)gene in pQE80L vector was mutated in position 26 GTG → GCG (Val 26 to Ala). The first methionine ATG codon was removed from t... |
restricted |
Olejnik Kamil |
Olejnik Kamil |
|
|
|
2808 |
BL21 Rosetta (DE3) |
E. coli |
Amp, Cam |
The wild type AtNUDT7 (construct 1791 P IBB Strain Colection)gene in pQE80L vector was mutated in position 69 GTT → GCT (Val 69 to Ala). The first methionine ATG codon was removed from t... |
restricted |
Olejnik Kamil |
Olejnik Kamil |
|
|
|
2809 |
BL21 Rosetta (DE3) |
E. coli |
Amp, Cam |
The Arabidopsis thaliana AtNUDT6 cDNA, 852 bp fragment, was cloned into MCS of pQE-80L vector using KpnI/PstI restriction sites. The first methionine ATG codon was removed. |
restricted |
Kamil Olejnik |
Kamil Olejnik |
|
|
|
2810 |
BL21 Rosetta (DE3) |
E. coli |
Amp, Cam |
The Arabidopsis thaliana AtNUDT6 cDNA, 852 bp fragment, was cloned into MCS of pGEX-4T-1 vector using EcoRI/SalI restriction sites. The first methionine ATG codon was removed. |
restricted |
Kamil Olejnik |
Kamil Olejnik |
|
|
|
2811 |
BL21 Rosetta (DE3) |
E. coli |
Amp, Cam |
The Arabidopsis thaliana AGG1 cDNA, 306 bp fragment was cloned into MCS of pQE-80L vector using KpnI/HindIII restriction sites. The first methionine ATG codon was removed. |
restricted |
Kamil Olejnik |
Kamil Olejnik |
|
|
|
2812 |
BL21 Rosetta (DE3) |
E. coli |
Amp, Cam |
The Arabidopsis thaliana AGG2 cDNA, 312 bp fragment was cloned into MCS of pQE-80L vector using KpnI/PstI restriction sites. The first methionine ATG codon was removed. |
restricted |
Kamil Olejnik |
Kamil Olejnik |
|
|
|
2813 |
BL21 Rosetta (DE3) |
E. coli |
Amp, Cam |
The wild type AtNUDT7 (construct 1791 P IBB Strain Colection)gene in pQE80L vector was mutated in position 73 TTT → GCT (Phe 73 to Ala). The first methionine ATG codon was removed from t... |
restricted |
Olejnik Kamil |
Olejnik Kamil |
|
|
|
2814 |
BL21 Rosetta (DE3) |
E. coli |
Amp, Cam |
The wild type AtNUDT7 (construct 1791 P IBB Strain Colection)gene in pQE80L vector was mutated in positions 73 TTT → GCT and 78 GCT → TTT (Phe 73 to Ala, Ala 78 to Phe). The first ... |
restricted |
Olejnik Kamil |
Olejnik Kamil |
|
|
|
2815 |
BL21 Rosetta (DE3) |
E. coli |
Amp, Cam |
The Arabidopsis thaliana RPP13 cDNA, 2508 bp fragment was cloned into MCS of pQE-82L vector using BamHI/PstI restriction sites. The first methionine ATG codon was removed. |
restricted |
Kamil Olejnik |
Kamil Olejnik |
|
|
|
2816 |
BL21 Rosetta (DE3) |
E. coli |
|
The 1020bp cDNA of NUDIX1 gene from P. ramorum (collection nr 2127) was cloned into pQE-80L expression vector using BamHI and PstI restriction sites. The 54 bp signal sequence from 5' end of t... |
restricted |
Olejnik Kamil |
Olejnik Kamil |
|
|
|
2925 |
IL1403:ΔhsdS |
Lactococcus lactis |
|
deletion of hsdS gene from the chromosome using pGhost9 integration/deletion method
|
restricted |
Roman Gorecki |
Roman Gorecki |
|
R. Górecki, PhD thesis |
|
2926 |
IL1403:ΔhsdRMS |
Lactococcus lactis |
|
deletion of hsdRMS genes from the chromosome using pGhost9 integration/deletion method
|
restricted |
Roman Gorecki |
Roman Gorecki |
|
R. Górecki, PhD thesis |
|
2927 |
PAO 1161 |
Pseudomonas aeruginosa |
Rif |
|
not restricted |
Magdalena Kusiak |
Magdalena Kusiak |
|
|
|
2928 |
PAO 1161 |
Pseudomonas aeruginosa |
Rif |
|
restricted |
Magdalena Kusiak |
Magdalena Kusiak |
|
|
|
2929 |
PAO 1161 |
Pseudomonas aeruginosa |
Rif |
|
not restricted |
Magdalena Kusiak |
Magdalena Kusiak |
|
|
|
2930 |
PAO 1161 |
Pseudomonas aeruginosa |
Rif |
|
not restricted |
Magdalena Kusiak |
Magdalena Kusiak |
|
|