| Base: |
Yeast strains |
| Institution Code: |
IBBPAS |
| Collection Code: |
ColIBB |
| Number in base: |
4017 |
| Species: |
Saccharomyces cerevisiae |
| Strain: |
SC80 |
| Genotype: |
MAT α his3Δ1 leu2Δ0 lys2Δ0 MET15 ura3Δ0 dpb2::kanMX4 [pAG1(pRS313 HIS3 dpb2-AG1)]
|
| Plasmid: |
pAG1 = pRS313 HIS3 dpb2-AG1 |
| Plasmid map: |
 |
| Selectable Markers: |
Leu Lys Ura kanMX4(G418) Amp |
| Antibiotic concentration: |
|
| Construction: |
Derivative of SC11 haploid strain (no.2893D); SC11 was transformed with pAG1; transformants were picked twice throught 5-FOA containing plates to lose pMJDPB2 (URA3) plasmid
|
| Strain properties/Comments: |
strain grows well at 23oC, not growing at 37oC; strain express mutator phenotype, dpb2-AG1 allele encodes mutated variant of Dpb2p with substitutions: Val84Ile Thr342Ile Ser343Phe Thr345Ile Pro347Ser Pro348Ser
|
| Author: |
Anna Gajda, Malgorzata Jaszczur |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Malgorzata Jaszczur |
| Deposition date: |
2009-03-04 18:03+01:00 |
| References: |
|