| Base: |
Yeast strains |
| Institution Code: |
IBBPAS |
| Collection Code: |
ColIBB |
| Number in base: |
4057 |
| Species: |
Saccharomyces cerevisiae |
| Strain: |
SC378 |
| Genotype: |
MATa trp1-289 his7-2 leu2-Δ::kanMX4 ura3-Δ ade2-1 lys2-ΔGG2899-2900 CAN1 pol2-4 dpb2::pKF117-DPB2(CaURA3, HIS3) rev3::CaURA3
|
| Plasmid: |
|
| Plasmid map: |
 |
| Selectable Markers: |
trp, lys, his, ade, kanMX4(G418), CAN1 |
| Antibiotic concentration: |
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| Construction: |
derivative of no. 4023 (SC94); SC94 was transformed with rev3::LEU2 disruption cassette; inetgration of rev3::LEU2 cassette leads to the deletion of the entire REV3 ORF; disruption confirmed by PCR
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| Strain properties/Comments: |
strain grows well at 23-37oC;mutation frequencies can be measured at different genetic loci; trp1-289,ade2-1 allow to measure base substitution; his7-2 lys2-ΔGG2899-2900 allow to measure frameshift mutations, CAN1 is a forward mutagenesis marker
|
| Author: |
Malgorzata Jaszczur |
| Availability: |
restricted |
| Patent: |
|
| Depositor: |
Malgorzata Jaszczur |
| Deposition date: |
2009-03-04 18:03+01:00 |
| References: |
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