Base: |
Plasmid collection |
Number in base: |
1642 |
Plasmid name: |
pPDB8.1 |
Species: |
Escherichia coli |
Strain: |
XL1-blue |
Genotype: |
recA1, endA1, gyrA96, thi-1, hsd R17, supE44, relA1, lac[F+ proAB lacIQZ ΔM15 Tn10(TetR)]
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Plasmid Sequence: |
non available
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Construction: |
pPET28mod(oriMB1,KmR, T7p, lacO, His tag; autor: Jagura-Burdzy) derrivative with EcoRI/SalI fragment carrying kfrC gene from R751, in frame
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Plasmid properties/Comments: |
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Plasmid map: |
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Selectable Markers: |
KanR |
Antibiotic concetration: |
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Plasmid size: |
~6kb |
Origin of replication/Incompatibility group: |
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Host range: |
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Other genetic elements: |
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Strain properties/Comments: |
fragment carrying kfrC from R751 was previously cloned in pGBT30 vector (EcoRI/SalI sites of MCS; this plasmid is called pMAB30.5, see references: Adamczyk, M., 2002, Ph.D.Thesis). EcoRI/SalI fragment of pMAB30.5 carrying kfrC has been inserted into EcoRI |
Author: |
Patrycja Dolowy |
Availability: |
non restricted |
Patent: |
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Depositor: |
Patrycja Dolowy |
Institution/Department: |
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Deposition date: |
2004-11-16 12:01+01:00 |
References: |
Adamczyk, M.(2002) The role of KfrA protein in the stable inheritance of IncP-1 plasmids. Ph.D. Thesis IBB PAN, warsaw, Poland |