| Base: |
Plasmid collection |
| Number in base: |
1643 |
| Plasmid name: |
pPDB8.3 |
| Species: |
Escherichia coli |
| Strain: |
DH5α |
| Genotype: |
recA1, endA1, gyrA96, thi-1, hsd R17(rK-mK+), supE44, relA1, deoR, Δlac(ZYA-argF), U169,[φ80Δ(lacM15)]
|
| Plasmid Sequence: |
non available
|
| Construction: |
pPET28mod(oriMB1,KmR, T7p, lacO, His tag; autor: Jagura-Burdzy) derrivative with EcoRI/SalI fragment carrying kfrB gene from R751, in frame
|
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
KanR |
| Antibiotic concetration: |
|
| Plasmid size: |
~5,8kb |
| Origin of replication/Incompatibility group: |
|
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
fragment carrying kfrB from R751 was previously cloned in pGBT30 vector (EcoRI/SalI sites of MCS; this plasmid is called pMAB30.4, see references: Adamczyk, M., 2002, Ph.D.Thesis). EcoRI/SalI fragment of pMAB30.4 carrying kfrB has been inserted into EcoRI |
| Author: |
Patrycja Dolowy |
| Availability: |
non restricted |
| Patent: |
|
| Depositor: |
Patrycja Dolowy |
| Institution/Department: |
|
| Deposition date: |
2004-11-16 12:01+01:00 |
| References: |
Adamczyk, M.(2002) The role of KfrA protein in the stable inheritance of IncP-1 plasmids. Ph.D. Thesis IBB PAN, Warsaw, Poland |