Base: |
Plasmid collection |
Number in base: |
1645 |
Plasmid name: |
pPDB1.17 |
Species: |
Escherichia coli |
Strain: |
C600 |
Genotype: |
thr-1 leu-6 thi-1 lacY1 supE44 tonA21 galK
|
Plasmid Sequence: |
non available
|
Construction: |
pPTOI(promotor probe vector: oriSC101, promotorless xylE, KmR; Thorsted et al., 1996) derrivative with BamHI/SphI fragment carrying klcA promotor region from RA3
|
Plasmid properties/Comments: |
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Plasmid map: |
|
Selectable Markers: |
KanR |
Antibiotic concetration: |
|
Plasmid size: |
~6kb |
Origin of replication/Incompatibility group: |
|
Host range: |
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Other genetic elements: |
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Strain properties/Comments: |
fragment carrying klcA promoter region was firstly amplified and cloned into pGEM- Teasy vector (called pPDB17). BamHI/SphI fragment of pPDB17 has been inserted into MCS of pPTOI vector, which is upstream the promotorless xylE gene |
Author: |
Patrycja Dolowy |
Availability: |
non restricted |
Patent: |
|
Depositor: |
Patrycja Dolowy |
Institution/Department: |
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Deposition date: |
2004-11-16 12:01+01:00 |
References: |
Thorsted, P. B., Shah, D. S., Macartney, D., Kostelidou, K., Thomas, C. M. (1996). Conservation og the genetic switch between replication and transfer genes of IncP plasmids but divergence of the replication functions which are major host-range determinat |