| Base: |
Plasmid collection |
| Number in base: |
1647 |
| Plasmid name: |
pPDB1.19 |
| Species: |
Escherichia coli |
| Strain: |
C600 |
| Genotype: |
thr-1 leu-6 thi-1 lacY1 supE44 tonA21 galK
|
| Plasmid Sequence: |
non available
|
| Construction: |
pPTOI(promotor probe vector: oriSC101, promotorless xylE, KmR; Thorsted et al., 1996) derrivative with BamHI/SphI fragment carrying promotor region of putative transposase from RA3
|
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
KanR |
| Antibiotic concetration: |
|
| Plasmid size: |
~6kb |
| Origin of replication/Incompatibility group: |
|
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
fragment carrying putative tnp promoter region was firstly amplified and cloned into pGEM- Teasy vector (called pPDB19). BamHI/SphI fragment of pPDB19 has been inserted into MCS of pPTOI vector, which is upstream the promotorless xylE gene |
| Author: |
Patrycja Dolowy |
| Availability: |
non restricted |
| Patent: |
|
| Depositor: |
Patrycja Dolowy |
| Institution/Department: |
|
| Deposition date: |
2004-11-16 12:01+01:00 |
| References: |
Thorsted, P. B., Shah, D. S., Macartney, D., Kostelidou, K., Thomas, C. M. (1996). Conservation og the genetic switch between replication and transfer genes of IncP plasmids but divergence of the replication functions which are major host-range determinat |