| Base: |
Plasmid collection |
| Number in base: |
1649 |
| Plasmid name: |
pPDB30.8 |
| Species: |
Escherichia coli |
| Strain: |
C600 |
| Genotype: |
thr-1 leu-6 thi-1 lacY1 supE44 tonA21 galK
|
| Plasmid Sequence: |
non available
|
| Construction: |
pGBT30 (oriMB1, ApR, lacIQ, tacp expression; Jagura-Burdzy et al, 1991) derrivative with EcoRI/SalI fragment encoding KorC protein of RA3
|
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
AmpR |
| Antibiotic concetration: |
|
| Plasmid size: |
~6,3kb |
| Origin of replication/Incompatibility group: |
|
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
DNA fragment carrying korC gene with additional EcoRI and SalI restriction sites was amlified and cloned in pGEM T-easy vector (oriMB1, ApR, Promega)and then EcoRI/SalI fragment from this construct was inserted into EcoRI/SalI sites in MCS of pGBT30 |
| Author: |
Patrycja Dolowy |
| Availability: |
non restricted |
| Patent: |
|
| Depositor: |
Patrycja Dolowy |
| Institution/Department: |
|
| Deposition date: |
2004-11-18 10:58+01:00 |
| References: |
Jagura-Burdzy, G., Ibbotson, J.P. and Thomas C.M. (1991) The korF region of broad-host-range plasmid RK2 encodes two polypeptides with trancriptional repressor activity. J Bacteriol 173: 826-833 |