Base: |
Plasmid collection |
Number in base: |
1651 |
Plasmid name: |
pPDB6.12 |
Species: |
Escherichia coli |
Strain: |
C600 |
Genotype: |
thr-1 leu-6 thi-1 lacY1 supE44 tonA21 galK
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Plasmid Sequence: |
non available
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Construction: |
pBTM116 (shuttle vector E. coli/ S. cerevisiae,oriMB1/2μ, trp1, LexABD, ApR; Invitrogen)derrivative with EcoRI/SalI fragment encoding KfrC from R751 with deletion of N-terminal part
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Plasmid properties/Comments: |
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Plasmid map: |
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Selectable Markers: |
AmpR |
Antibiotic concetration: |
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Plasmid size: |
~6,1kb |
Origin of replication/Incompatibility group: |
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Host range: |
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Other genetic elements: |
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Strain properties/Comments: |
Fragment carrying kfrC gene was previously amlified and cloned into pGAD424 vector (shuttle vector, see references: Adamczyk, M., 2002, Ph.D.Thesis - pMAB424.5). pMAB424.5 was cleaved with XmaIII and blut-ended with Klenow fragment of polymerase then liga |
Author: |
Patrycja Dolowy |
Availability: |
non restricted |
Patent: |
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Depositor: |
Patrycja Dolowy |
Institution/Department: |
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Deposition date: |
2004-11-18 10:58+01:00 |
References: |
Adamczyk, M.(2002) The role of KfrA protein in the stable inheritance of IncP-1 plasmids. Ph.D. Thesis IBB PAN, Warsaw, Poland |