| Base: |
Plasmid collection |
| Number in base: |
1652 |
| Plasmid name: |
pPDB4.12 |
| Species: |
Escherichia coli |
| Strain: |
C600 |
| Genotype: |
thr-1 leu-6 thi-1 lacY1 supE44 tonA21 galK
|
| Plasmid Sequence: |
non available
|
| Construction: |
pGAD424 (shuttle vector E. coli/ S. cerevisiae,oriMB1/2μ, leu2, GAL4AD, ApR; Fields and Song, 1989)derrivative with EcoRI/SalI fragment encoding KfrC from R751 with deletion of N-terminal part
|
| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
AmpR |
| Antibiotic concetration: |
|
| Plasmid size: |
~6,9kb |
| Origin of replication/Incompatibility group: |
|
| Host range: |
|
| Other genetic elements: |
|
| Strain properties/Comments: |
Fragment carrying kfrC gene was previously amlified and cloned into pGAD424 vector (shuttle vector, see references: Adamczyk, M., 2002, Ph.D.Thesis - pMAB424.5). pMAB424.5 was cleaved with XmaIII and blut-ended with Klenow fragment of polymerase then liga |
| Author: |
Patrycja Dolowy |
| Availability: |
non restricted |
| Patent: |
|
| Depositor: |
Patrycja Dolowy |
| Institution/Department: |
|
| Deposition date: |
2004-11-18 10:58+01:00 |
| References: |
1)Fields, S and Song, O. (1989) A novel genetic system to detect protein-protein interaction. Nature 340: 245-246; 2)Adamczyk, M.(2002) The role of KfrA protein in the stable inheritance of IncP-1 plasmids. Ph.D. Thesis IBB PAN, Warsaw, Poland |