| Base: |
Plasmid collection |
| Number in base: |
1654 |
| Plasmid name: |
pPDB4.32 |
| Species: |
Escherichia coli |
| Strain: |
DH5α |
| Genotype: |
recA1, endA1, gyrA96, thi-1, hsd R17(rK-mK+), supE44, relA1, deoR, Δlac(ZYA-argF), U169,[φ80Δ(lacM15)]
|
| Plasmid Sequence: |
non available
|
| Construction: |
pGAD424 (shuttle vector E. coli/ S. cerevisiae,oriMB1/2μ, leu2, GAL4AD, ApR; Fields and Song, 1989)derrivative with EcoRI/SalI fragment encoding KfrB from R751 with deletion of N-terminal part
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| Plasmid properties/Comments: |
|
| Plasmid map: |
 |
| Selectable Markers: |
AmpR |
| Antibiotic concetration: |
|
| Plasmid size: |
~6,8kb |
| Origin of replication/Incompatibility group: |
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| Host range: |
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| Other genetic elements: |
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| Strain properties/Comments: |
Fragment carrying kfrB gene was previously amlified and cloned into pGAD424 vector (shuttle vector, see references: Adamczyk, M., 2002, Ph.D.Thesis - pMAB424.4). pMAB424.4 was cleaved with XmaIII and blut-ended with Klenow fragment of polymerase then liga |
| Author: |
Patrycja Dolowy |
| Availability: |
non restricted |
| Patent: |
|
| Depositor: |
Patrycja Dolowy |
| Institution/Department: |
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| Deposition date: |
2004-11-18 10:58+01:00 |
| References: |
1) Fields, S and Song, O. (1989) A novel genetic system to detect protein-protein interaction. Nature 340: 245-246; 2) Adamczyk, M.(2002) The role of KfrA protein in the stable inheritance of IncP-1 plasmids. Ph.D. Thesis IBB PAN, Warsaw, Poland |