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Central Collection of Strains (IBB)

Base: Plasmid collection
Number in base: 1663
Plasmid name: pPDB4.13
Species: Escherichia coli
Strain: DH5α
Genotype: recA1, endA1, gyrA96, thi-1, hsd R17(rK-mK+), supE44, relA1, deoR, Δlac(ZYA-argF), U169,[φ80Δ(lacM15)]
Plasmid Sequence: non available
Construction: The EcoRI-SalI fragment carrying kfrA of RK2 inserted into YTH system vector pGAD424 to create GAL4AD-KfrARK2 translational fusion
Plasmid properties/Comments:
Plasmid map:
Selectable Markers: Amp
Antibiotic concetration:
Plasmid size: ~7,8kb
Origin of replication/Incompatibility group:
Host range:
Other genetic elements:
Strain properties/Comments:
Author: Patrycja Dolowy
Availability: non restricted
Patent:
Depositor: Patrycja Dolowy
Institution/Department:
Deposition date: 2006-06-29 14:49+01:00
References: Jagura-Burdzy, G. and Thomas, C.M.(1992) kfrA gene of broad host range plasmid RK2 encodes a novel DNA-binding protein. J.Mol.Biol. (225)